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Sarai, Akinori*; Ueno, Takuya*; Ngahu, A.*; Ahmad, S.*; Kono, Hidetoshi
no journal, ,
no abstracts in English
Fujii, Satoshi*; Ara
zo-Bravo, M. J.*; Takenaka, Shigeori*; Kono, Hidetoshi; Go, Nobuhiro; Sarai, Akinori*
no journal, ,
no abstracts in English
HA1-2mAb Fab) with antigen peptideArai, Shigeki; Tamada, Taro; Okamoto, Yoshiko*; Hifumi, Megumi*; Uda, Taizo*; Kuroki, Ryota
no journal, ,
no abstracts in English
Tamada, Taro; Kinoshita, Takayoshi*; Kuroki, Ryota; Tada, Toshiji*
no journal, ,
no abstracts in English
Adachi, Motoyasu; Tamada, Taro; Sato, Katsuya; Yura, Kei; Narumi, Issei; Kuroki, Ryota
no journal, ,
Deinococcus radiodurans exhibits 1000 times higher radiation resistance than human cells. PprA is cloned from the bacteria and the novel DNA repair protein that plays an important role in the resistance. This study is aimed to reveal the structure function relationship of PprA. The recombinant PprA protein was purified from E. coli. The interactions of PprA with DNA were analyzed by gel shift assay and gel filtration. The results showed that (1) at least 280 molecules of PprA can bound to a DNA molecule of pUC19 composed of 2686bp (2) the formation the complex of PprA and DNA depends on Mg, Ca and Sr ions at low concentration (3) the formation the complex of PprA and DNA depends on the concentration of the salt at the range of 0 to 0.4M sodium acetate. In addition, the result indicated that the complexes of PprA and DNA can form larger complex depending on the concentration of PprA molecule, when using linear double stranded DNA. This suggests that PprA may locate two terminals of a DNA molecule damaged by radiation at near distance for DNA repairing.
Kuroki, Ryota; Honjo, Eijiro; Tamada, Taro
no journal, ,
Silk worm expresssion system is the rapid system for several protein expressions developed by Katakura Industries company. We have used this system for test expression of the extracellular region of receptors. Every cDNA coding extracellular region of receptors were fused to the cDNA of Fc region of antibody, and cloned into the transfer vector and the vector was transformed into vacuro virus that can infect silk worm. So far, expression of 10 extracellular regions of menbrane proteins were tested using this system, all proteins were successfully expressed. Among these proteins, interleukin-13 receptor alpha 1 and 2, interluekin-4 receptor were purified from the silk worm and their functions were comfirmed.
Yura, Kei
no journal, ,
no abstracts in English
Adachi, Motoyasu; Tamada, Taro; Sato, Katsuya; Yura, Kei; Narumi, Issei; Kuroki, Ryota
no journal, ,
no abstracts in English
Honjo, Eijiro; Tamada, Taro; Ikura, Teikichi*; Kuroki, Ryota
no journal, ,
no abstracts in English